Ribosome binding site prediction software

It is used to design synthetic biological systems. Log in to Open Hub. Remember Me. Analyzed about 21 hours ago. Project Summary The Ribosome Binding Site RBS Calculator can predict the translation initiation rate of a protein coding sequence in bacteria and design synthetic RBS sequences to rationally control the translation initiation rate.

Tags No tags have been added. Quick Reference. Project Links:. Code Locations:. The method may also optimize a synthetic RBS sequence to achieve a targeted translation initiation rate.

We begin by providing an overview of the potential biotechnology applications of the RBS Calculator, including the optimization of synthetic metabolic pathways and genetic circuits.

We then detail the definitions, methodologies, and algorithms behind the RBS Calculator's thermodynamic model and optimization method. Article Navigation. Close mobile search navigation Article Navigation. Volume Article Contents Abstract. RBSDesigner : software for designing synthetic ribosome binding sites that yields a desired level of protein expression.

Dokyun Na , Dokyun Na. Oxford Academic. Associate Editor: Martin Bishop. Revision received:. Select Format Select format. Permissions Icon Permissions. Open in new tab Download slide. De Smit. Secondary structure of the ribosome binding site determines translational efficiency: a quantitative analysis. Google Scholar Crossref. Search ADS. Strategies for achieving high-level expression of genes in Escherichia coli. Google Scholar PubMed. Construction and optimization of synthetic pathways in metabolic engineering.

Translation initiation in Escherichia coli: sequences within the ribosome-binding site. Automated design of synthetic ribosome binding sites to control protein expression. Artificial genetic selection for an efficient translation initiation site for expression of human RACK1 gene in Escherichia coli. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.

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